WebJun 23, 2024 · Open the culture plate with the opposite hand and press the tape against the colony of interest. Place a drop of Lactophenol Blue on a clean glass slide. Press tape … WebPlace a drop of lactophenol cotton blue stain on a clean microscope slide. Remove the cover glass from the slide culture and discard the block of agar. Add a drop of 95% ethanol to the hyphae on the cover glass. As soon as most of the alcohol has evaporated place the cover glass, mold side down, on the drop of lactophenol cotton blue stain on ...

University of Wisconsin–Milwaukee

WebCotton blue (Poirier blue or Aniline blue): 0.025 g. Distilled water : 10 ml. Dissolve phenol in lactic acid, glycerol, and distilled water. Finally, add cotton blue and mix well. But this LPCB stain is prepared over two days. On the first day, dissolve the cotton blue in the distilled water and leave it overnight to eliminate insoluble dye. WebLactophenol cotton blue (LCB) is a mixture of methyl blue, a histological stain, and lactophenol (a solution of phenol, lactic acid, and glycerol in water). It is used in wet … shotleft free state

National Center for Biotechnology Information

WebThis stain is prepared over two days. On the first day, dissolve the Cotton blue in the distilled water. Leave overnight to eliminate insoluble dye. On the second day, wearing gloves add the phenol crystals to the lactic acid in a glass beaker. place on magnetic stirrer until the phenol is dissolved. Add the glycerol. WebProduct name : Lactophenol (Co tton Blue) Solution 1.2 Relevant identified uses of the substance or mixture and uses advised against Identified uses : Laboratorychemicals, … WebOct 30, 2014 · Lactophenol cotton blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic … sargent corporation podcast