WebDec 10, 2024 · This article provides an informed reality check about the utility and dangers of, and myths surrounding, ethidium bromide. ... It emits a red/orange light at an emission maximum of 560 nm upon excitation with ultraviolet light (maxima ranging from 260 nm to 360 nm). ... Development of a SYBR safe technique for the sensitive detection of DNA in ... WebEthidium bromide is a sensitive, easy stain for DNA. It yields low background and a detection limit of 1-5 ng /band. The major drawback to ethidium bromide is that it is a potent mutagen....
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molecular biology - Just how light-sensitive is ethidium …
Webthat fluoresce when 300 nm UV is used to transmit light through the gel (e.g., ethidium bromide, SYBR Green, SYPRO Red and Orange, etc). This generally requires that the gel or sample be transparent or semi-transparent, as opposed to opaque. When using UV applications, disconnect the white light pad from the AC port and store WebThe products of restriction enzyme cleavage were analyzed on 2% agarose gels and were visualized under UV light after staining with ethidium bromide. Alleles ‘G’ and ‘T’ were assigned based on the presence of a 740 bp (uncleaved) fragment and the 528 bp and 210 bp (cleaved) fragments, respectively ( Figure 1 ). WebApr 12, 2024 · Add 8 μL ethidium bromide (see Note 16) to the buffer beyond with care to avoid the gel area (see Note 17). Turn on the orbital shaker and allow to stain for 20 min. 10. Transfer the gel to a gel documentation system (gel doc). Turn on the gel doc and the UV lamp to excite the ethidium bromide-stained amplified products on the gel. 11. bottle making company